4KG9

Crystal Structure Of USP7-NTD with MCM-BP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-25
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	69.799, 69.799, 45.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.710 - 1.700
R-factor	0.206
Rwork	0.206
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy6
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	20711
<I/σ(I)>	21.9	1.6
Redundancy	11.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	30 % PEG 4000, 0.1 M Tris, 0.2 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277KK, pH 8.5