4KG4

Crystal structure of Saccharomyces cerevisiae Dcp2 Nudix domain (E198Q mutation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-23
Wavelength(s)	1.11587
Spacegroup name	P 1 21 1
Unit cell lengths	57.352, 47.804, 60.957
Unit cell angles	90.00, 97.29, 90.00

Refinement procedure

Resolution	44.336 - 1.800
R-factor	0.1889
Rwork	0.187
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.166
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.040	0.028	0.383
Number of reflections	29367
<I/σ(I)>	18.6
Completeness [%]	96.3	89.9	97.1
Redundancy	1.8	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	10 mM HEPES, 1 mM DTT, 50 mM NaCl, 100 mM Na2SO4, 0.25 M Na Acetate, 20% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K