4KG4
Crystal structure of Saccharomyces cerevisiae Dcp2 Nudix domain (E198Q mutation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-23 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.352, 47.804, 60.957 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 44.336 - 1.800 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.166 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.040 | 0.028 | 0.383 |
| Number of reflections | 29367 | ||
| <I/σ(I)> | 18.6 | ||
| Completeness [%] | 96.3 | 89.9 | 97.1 |
| Redundancy | 1.8 | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10 mM HEPES, 1 mM DTT, 50 mM NaCl, 100 mM Na2SO4, 0.25 M Na Acetate, 20% PEG 3350, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
