4KFW

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Detector technology	CCD
Collection date	2012-07-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	83.077, 83.077, 145.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.810 - 2.700
R-factor	0.2067
Rwork	0.202
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	2.700	6.890	2.700
Number of reflections	14443
Completeness [%]	98.2	98.1	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8.5	293	0.1M Tris (pH 8.5) 25% PEG3350 0.2 M (NH4)2Ac, LIQUID DIFFUSION, temperature 293K