4KFR
Structure of the genome packaging NTPase B204 from Sulfolobus turreted icosahedral virus 2 in complex with sulfate
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979790,0.979860,0.979110 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.571, 59.604, 77.561 |
Unit cell angles | 90.00, 111.08, 90.00 |
Refinement procedure
Resolution | 48.587 - 1.956 |
R-factor | 0.1796 |
Rwork | 0.178 |
R-free | 0.21600 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.142 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.587 | 2.070 |
High resolution limit [Å] | 1.956 | 1.956 |
Rmerge | 0.057 | 0.528 |
Number of reflections | 58758 | |
<I/σ(I)> | 14 | 2.15 |
Completeness [%] | 98.0 | 96.6 |
Redundancy | 3.17 | 3.17 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1 M sodium cacodylate, 0.21 M ammonium sulfate, 0.05 M magnesium chloride, protein in 50 mM sodium citrate, 35% PEG6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |