4KF8
Nup188(aa1445-1827) from Myceliophthora thermophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.298, 66.651, 162.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.131 - 3.000 |
| R-factor | 0.2769 |
| Rwork | 0.275 |
| R-free | 0.29420 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.769 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.131 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.027 | 0.561 |
| Number of reflections | 14539 | |
| <I/σ(I)> | 21.4 | 4.8 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 18-23% w/v PEG3350, 150 mM tri-ammonium citrate, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
