4KEK

Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant R176Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2008-02-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	I 41
Unit cell lengths	100.272, 100.272, 37.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.984 - 2.146
R-factor	0.2517
Rwork	0.250
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3im7
RMSD bond length	0.002
RMSD bond angle	0.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.146	5.830	2.146
Number of reflections	10342
Completeness [%]	98.1	91.1	86
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K