4KEI

Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant P164S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Detector technology	CCD
Collection date	2009-12-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97949
Spacegroup name	I 41
Unit cell lengths	111.745, 111.745, 37.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.337 - 2.406
R-factor	0.2438
Rwork	0.242
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3im7
RMSD bond length	0.002
RMSD bond angle	0.554
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Number of reflections	9226
<I/σ(I)>	45.24
Completeness [%]	99.4	98.3	96
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	0.2 M sodium malonate, 5-15% saturated ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K