4KEF
Structure of Cofilin Mutant (cof1-159p)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.096, 69.544, 32.048 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 28.697 - 1.098 |
| R-factor | 0.1272 |
| Rwork | 0.127 |
| R-free | 0.14430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.140 |
| High resolution limit [Å] | 1.098 | 1.098 |
| Number of reflections | 49213 | |
| <I/σ(I)> | 2.8 | 2.8 |
| Completeness [%] | 99.8 | 98.99 |
| Redundancy | 4.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 20% PEG4000, 150mM NaCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
