4KEE
Crystal Structure of Cofilin Mutant (cof1-158p)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.119, 69.852, 32.164 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 14.942 - 1.448 |
| R-factor | 0.1391 |
| Rwork | 0.135 |
| R-free | 0.17480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.448 | 1.450 |
| Number of reflections | 20869 | |
| <I/σ(I)> | 2.7 | 2.7 |
| Completeness [%] | 96.0 | 72.34 |
| Redundancy | 6.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 20% PEG 4000, 150mM NaCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
