4KDV
Crystal structure of a bacterial immunoglobulin-like domain from the M. primoryensis ice-binding adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-12 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.690, 43.020, 32.260 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 25.700 - 2.420 |
| R-factor | 0.149 |
| Rwork | 0.144 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.700 | 2.570 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.079 | 0.095 |
| Number of reflections | 5343 | |
| <I/σ(I)> | 15.36 | 11.15 |
| Completeness [%] | 91.0 | 80.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 298 | 0.2M calcium acetate, 0.1 M sodium acetate and 30% (v/v) PEG, microbatch, temperature 298.0K , pH 4.5, EVAPORATION |
