4KCA
Crystal Structure of Endo-1,5-alpha-L-arabinanase from a Bovine Ruminal Metagenomic Library
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.429 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.909, 114.318, 167.201 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.840 - 1.900 |
R-factor | 0.18846 |
Rwork | 0.187 |
R-free | 0.21565 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.637 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 147570 | |
Completeness [%] | 98.3 | 94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Sodium Malonate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |