4KBM

Structure of the Mtb CarD/RNAP Beta subunit B1-B2 domains complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-09
Detector	MAR 300
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	49.152, 128.889, 225.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.588 - 2.115
R-factor	0.2095
Rwork	0.208
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kbj
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (Phaser)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.590	2.210
High resolution limit [Å]	2.110	2.110
Number of reflections	40844
<I/σ(I)>		2.03
Completeness [%]	98.1	92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	14% PEG 3350, 2% tacsimate pH 5.0, 0.1 M sodium citrate tribasic dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 291K