4KBG
almost closed conformation of the helicase core of the RNA helicase Hera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99997 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 127.200, 128.702, 101.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.348 - 2.540 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs 2db3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1327)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.240 | 2.640 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 27922 | |
| <I/σ(I)> | 8.19 | 1.03 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.66 | 6.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | VAPOR DIFFUSION, SITTING DROP, temperature 293K |
