4KBF
two different open conformations of the helicase core of the RNA helicase Hera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-04-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 119.645, 119.645, 107.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.876 - 1.900 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxq 2db3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1327)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 61258 | |
| <I/σ(I)> | 18.7 | 1.58 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 13.01 | 12.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | VAPOR DIFFUSION, SITTING DROP, temperature 293K |
