4KB5
Crystal structure of MycP1 from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-21 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97914 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 85.114, 85.114, 193.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.18955 |
Rwork | 0.188 |
R-free | 0.21410 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.890 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.071 | 0.507 |
Number of reflections | 39312 | |
<I/σ(I)> | 25.9 | 4.1 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 7.6 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 283 | 1.6 M Magnesium sulfate, 0.1 M MES pH 5.5 with a protein concentration of 20 mg ml-1, VAPOR DIFFUSION, SITTING DROP, temperature 283K |