4KB4
Crystal structure of ribosome recycling factor mutant R31A from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.514 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.200, 40.450, 68.540 |
| Unit cell angles | 90.00, 118.41, 90.00 |
Refinement procedure
| Resolution | 46.240 - 2.250 |
| R-factor | 0.21812 |
| Rwork | 0.216 |
| R-free | 0.26385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.697 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.300 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.087 | 0.087 |
| Number of reflections | 11114 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil in microbatch plates | 7.8 | 298 | 6% Polyethylene glycol 4000, 20mM Tris-HCl, 0.6mM cadmium acetate, 5% polyethylene glycol 400, pH 7.8, Under oil in microbatch plates, temperature 298KK |
