4KB2
Crystal structure of ribosome recycling factor mutant R109A from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-01-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.490, 33.740, 61.150 |
| Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
| Resolution | 51.970 - 2.300 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.26686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.212 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.200 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.165 | 0.165 |
| Number of reflections | 9680 | |
| <I/σ(I)> | 6.9 | 6.9 |
| Completeness [%] | 98.6 | |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil using microbatch plates | 7.8 | 298 | 6% Polyethylene glycol 4000, 20mM Tris-HCl (pH 7.8), 0.6mM Cadmium acetate, 5% Polyethylene glycol 400, Under oil using microbatch plates, temperature 298K |
