4KA4
Crystal structure of a proteolytically defined Zbeta domain of human DAI (ZBP1, DLM-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.647, 63.208, 94.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 2.600 |
| R-factor | 0.2529 |
| Rwork | 0.248 |
| R-free | 0.30170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyi |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.550 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.430 |
| Number of reflections | 10291 | |
| <I/σ(I)> | 12.1 | 3.6 |
| Completeness [%] | 99.1 | 96.4 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 8% PEG 8000, 0.1M sodium acetate, 20% glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
