4KA4
Crystal structure of a proteolytically defined Zbeta domain of human DAI (ZBP1, DLM-1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.647, 63.208, 94.541 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.270 - 2.600 |
R-factor | 0.2529 |
Rwork | 0.248 |
R-free | 0.30170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eyi |
RMSD bond length | 0.001 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.270 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.078 | 0.430 |
Number of reflections | 10291 | |
<I/σ(I)> | 12.1 | 3.6 |
Completeness [%] | 99.1 | 96.4 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 8% PEG 8000, 0.1M sodium acetate, 20% glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |