4K9R
Spore photoproduct lyase Y98F mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.020, 62.720, 142.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.060 - 2.300 |
R-factor | 0.21593 |
Rwork | 0.213 |
R-free | 0.26418 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.060 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.132 | 0.674 |
Number of reflections | 22170 | |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 200 mM Li-sulfate, 100 mM Tris/HCl, 19-27% PEG8000, 70 mM HEGA-8, anaerobically, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |