4K8W
An arm-swapped dimer of the S. pyogenes pilin specific assembly factor SipA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.285, 53.285, 131.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.310 - 1.670 |
| R-factor | 0.18662 |
| Rwork | 0.185 |
| R-free | 0.21460 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.310 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.043 | 0.262 |
| Number of reflections | 22923 | |
| <I/σ(I)> | 12.3 | 13.3 |
| Completeness [%] | 100.0 | 96.9 |
| Redundancy | 35.3 | 30.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15 % ethanol, 0.10 M Tris.Cl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
