4K8I
Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | CCD |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 47.200, 47.200, 63.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.120 - 2.100 |
R-factor | 0.20007 |
Rwork | 0.198 |
R-free | 0.24705 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.263 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.130 | 1.650 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.062 | 0.702 |
Number of reflections | 26865 | |
<I/σ(I)> | 0.6 | |
Redundancy | 1.8 | 1.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25mM Sodium Phosphate, MPD 35-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |