4K82
Crystal structure of lv-ranaspumin (Lv-RSN-1) from the foam nest of Leptodactylus vastus, monoclinic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.874, 25.848, 66.076 |
Unit cell angles | 90.00, 103.98, 90.00 |
Refinement procedure
Resolution | 25.629 - 1.600 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.23730 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.119 | 45.259 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.060 | 0.781 | |
Total number of observations | 3721 | 18307 | |
Number of reflections | 22985 | ||
<I/σ(I)> | 9.8 | 9.1 | 1 |
Completeness [%] | 99.9 | 96.3 | 100 |
Redundancy | 5.5 | 4.9 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapour diffusion, sitting drop | 7.3 | 293 | 0.2 M Potassium formate, 20% (w/v) PEG 3350, pH 7.3, vapour diffusion, sitting drop, temperature 293K |