4K7P
Generation and Characterization of a Unique Reagent that Recognizes a Panel of Recombinant Human Monoclonal Antibody Therapeutics in the Presence of Endogenous Human IgG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.917, 109.099, 119.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.22184 |
| Rwork | 0.219 |
| R-free | 0.28402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.043 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 25377 |
| <I/σ(I)> | 11 |
| Completeness [%] | 98.8 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | 200 mM NaCl, phosphate buffer, PEG1000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
