4K79
Recognition of the Thomsen-Friedenreich Antigen by a Lamprey Variable Lymphocyte Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.680, 68.680, 99.920 |
| Unit cell angles | 90.00, 93.58, 90.00 |
Refinement procedure
| Resolution | 33.660 - 2.200 |
| R-factor | 0.20129 |
| Rwork | 0.201 |
| R-free | 0.25142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6j |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.657 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.660 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 38816 | |
| Completeness [%] | 99.1 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% (w/v) polyethylene glycol (PEG) 3350, 4% (v/v) glycerol, and 0.1M Tris-HCl (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
