4K6N
Crystal structure of yeast 4-amino-4-deoxychorismate lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97838 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.966, 113.893, 86.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.790 - 1.900 |
R-factor | 0.1744 |
Rwork | 0.173 |
R-free | 0.20180 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.079 | 0.422 |
Number of reflections | 35258 | |
<I/σ(I)> | 20 | 4.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289.15 | 20%(w/v) polyethylene glycol monomethyl ether 5000, 0.1M Bis-Tris, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |