4K6E

Crystal structure of Saccharomyces cerevisiae Dcp2 Nudix domain in complex with Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	298
Detector technology	CCD
Collection date	2011-04-23
Spacegroup name	P 62
Unit cell lengths	85.054, 85.054, 48.749
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.527 - 2.100
R-factor	0.1568
Rwork	0.153
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.085
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.081	0.032	0.557
Number of reflections	11889
<I/σ(I)>	8.3
Completeness [%]	99.9	100	99.7
Redundancy	7.3	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	10 mM HEPES, 1 mM DTT, 50 mM NaCl, 100 mM Na2SO4, 0.1 M Mg Formate, 20% Peg 3350, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K