4K6B

Atomic structure of bacteriophage HS1 tail needle knob

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	77
Detector technology	CCD
Collection date	2008-11-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	56.780, 87.363, 88.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.976 - 1.101
R-factor	0.1514
Rwork	0.151
R-free	0.15700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.362
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.100
Number of reflections	176986
<I/σ(I)>	29.96
Completeness [%]	99.1
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	26% PEG 3350, 50mM TRIS-HCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	26% PEG 3350, 50mM TRIS-HCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K