4K68
Structure of a novel GH10 endoxylanase retrieved from sugarcane soil metagenome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.459 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.877, 116.442, 82.811 |
Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
Resolution | 41.200 - 2.740 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.588 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.200 | 2.850 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.143 | 0.468 |
Number of reflections | 20118 | |
Completeness [%] | 97.6 | 87.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | PEG8000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K |