4K63

Structure of an avian influenza H5 hemagglutinin from the influenza virus complexed with avian receptor analog LSTa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 3
Unit cell lengths	70.555, 70.555, 506.295
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.490 - 3.100
R-factor	0.2481
Rwork	0.245
R-free	0.30690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fk0
RMSD bond length	0.004
RMSD bond angle	1.082
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	42807
Completeness [%]	82.0	83.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	25% PEG 2000 MME, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K