4K59
Crystal Structure of Pseudomonas Aeruginosa RsmF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 41.451, 41.451, 74.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.302 - 2.220 |
| R-factor | 0.2257 |
| Rwork | 0.221 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
| High resolution limit [Å] | 2.220 | 6.020 | 2.220 |
| Rmerge | 0.049 | 0.039 | 0.368 |
| Number of reflections | 3743 | ||
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 98.8 | 96.7 | 93.9 |
| Redundancy | 3.9 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 30% PEG 4000, 0.2 M Magnesium chloride, 0.1 M Tris-HCl, pH 8.5, vapor diffusion, temperature 298K |
