4K4Z
Coxsackievirus B3 polymerase elongation complex (r2_Mg_form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | NOIR-1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.641, 60.633, 194.680 |
| Unit cell angles | 89.98, 89.92, 78.68 |
Refinement procedure
| Resolution | 45.580 - 2.170 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | d*TREK (9.9.9.4D) |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.055 | 0.474 |
| Number of reflections | 140661 | |
| <I/σ(I)> | 7.7 | 1.5 |
| Completeness [%] | 97.8 | 97.1 |
| Redundancy | 1.95 | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | sitting drop, temperature 289K, VAPOR DIFFUSION, SITTING DROP |
