4K4V
Poliovirus polymerase elongation complex (r5+1_form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 63.519, 63.475, 101.946 |
Unit cell angles | 73.48, 73.44, 73.64 |
Refinement procedure
Resolution | 47.506 - 2.630 |
R-factor | 0.2349 |
Rwork | 0.233 |
R-free | 0.27280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.287 |
Data reduction software | d*TREK (9.9.8.0L) |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.510 | 47.510 | 2.720 |
High resolution limit [Å] | 2.630 | 5.660 | 2.630 |
Rmerge | 0.042 | 0.031 | 0.448 |
Total number of observations | 8332 | 8305 | |
Number of reflections | 41557 | ||
<I/σ(I)> | 8.4 | 28 | 1.5 |
Completeness [%] | 98.0 | 96.5 | 97.7 |
Redundancy | 1.99 | 1.97 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 4.8%(v/v) isopropanol, 0.096 M tacsimate (Hampton Research), 1.92-1.95 M ammonium sulfate, and 10-11%(v/v) glycerol and then gradually exchanged into a cryo stabilizer solution containing 4.8%(v/v) isopropanol, 0.096 M tacsimate, 1.95 M ammonium sulfate and 19-27% (v/v) xylitol with dCTP prior to freezing, pH pH 8.5-9.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |