4K3H
Immunoglobulin lambda variable domain L5(L89S) fluorogen activationg protein in complex with malachite green
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 43 |
| Unit cell lengths | 94.110, 94.110, 175.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 2.450 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.167 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.180 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.073 | 0.506 |
| Number of reflections | 56006 | |
| <I/σ(I)> | 20.5 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295 | 0.29M ammonium sulfate, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
