4K3A

The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	84.875, 91.758, 92.819
Unit cell angles	90.00, 98.60, 90.00

Refinement procedure

Resolution	31.399 - 2.300
R-factor	0.1523
Rwork	0.150
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k35
RMSD bond length	0.009
RMSD bond angle	1.195
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	61059
Completeness [%]	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG4000, Tris, MPD, lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K