4K38
Native anSMEcpe with bound AdoMet and Kp18Cys peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.383, 92.068, 91.102 |
| Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
| Resolution | 41.085 - 1.831 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.20540 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.456 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.830 |
| Number of reflections | 64439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 29% PEG 4000, 150 mM ammonium acetate, 100 mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 298K |
