4K2X
OxyS anhydrotetracycline hydroxylase from Streptomyces rimosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.060, 115.130, 121.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.050 - 2.550 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fmw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.050 | 11.400 | 2.620 |
High resolution limit [Å] | 2.550 | 8.060 | 2.550 |
Rmerge | 0.081 | 0.034 | 0.507 |
Number of reflections | 31457 | 667 | 2214 |
<I/σ(I)> | 13.58 | 29.76 | 3.37 |
Completeness [%] | 89.0 | 86 | 85.6 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 16% PEG-8000, 0.1M Na/K phosphate pH 6.0, 0.2M NaCl, vapor diffusion, hanging drop, temperature 298K |