4K2U
Crystal structure of PfEBA-175 F1 in complex with R218 antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791212 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 101.950, 53.530, 156.790 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 29.793 - 2.450 |
| R-factor | 0.2057 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.609 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.793 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 66216 | |
| Completeness [%] | 98.8 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 290 | 0.1 M phosphate-citrate, 0.4 M lithium sulfate, 30% PEG 1000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
