4K2C
HSA Ligand Free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.410, 179.888, 57.459 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 47.700 - 3.230 |
| R-factor | 0.21572 |
| Rwork | 0.213 |
| R-free | 0.26507 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.030 | 3.030 |
| Number of reflections | 18979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 25-30% PEG 3350, pH 7, VAPOR DIFFUSION, temperature 298K |
