4K2C
HSA Ligand Free
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.04 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.410, 179.888, 57.459 |
Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
Resolution | 47.700 - 3.230 |
R-factor | 0.21572 |
Rwork | 0.213 |
R-free | 0.26507 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.474 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.030 | 3.030 |
Number of reflections | 18979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 25-30% PEG 3350, pH 7, VAPOR DIFFUSION, temperature 298K |