4K1O

Crystal structure of the alphaN-catenin actin-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-15
Detector	ADSC Q210
Wavelength(s)	0.97911
Spacegroup name	P 32 1 2
Unit cell lengths	112.152, 112.152, 56.316
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.736 - 2.603
R-factor	0.1982
Rwork	0.195
R-free	0.23090
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.104
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge		0.031	0.519
Number of reflections	12361
<I/σ(I)>	16.9
Completeness [%]	99.7	95.6	100
Redundancy	9	8.4	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	0.1M HEPES, pH 7.0, 1.6 M (NH4)2SO4, 0.2 M NaCl,and 0.1M K/Na tartate, vapor diffusion, temperature 277K
1	VAPOR DIFFUSION	7	277	0.1M HEPES, pH 7.0, 1.6 M (NH4)2SO4, 0.2 M NaCl,and 0.1M K/Na tartate, vapor diffusion, temperature 277K