4K1N
Crystal structure of full-length mouse alphaE-catenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 |
| Unit cell lengths | 144.684, 144.684, 136.757 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 6.500 |
| Rwork | 0.224 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DOV and 1ST6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.813 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 6.610 |
| High resolution limit [Å] | 6.500 | 17.400 | 6.500 |
| Rmerge | 0.093 | 0.521 | |
| Number of reflections | 6581 | ||
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.7 | 95 | 100 |
| Redundancy | 5.2 | 4.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Sodium Citrate, 20% (w/v) PEG-3350, vapor diffusion, temperature 293K | |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Sodium Citrate, 20% (w/v) PEG-3350, vapor diffusion, temperature 293K |
