4K14
Crystal Structure of Staphylococcal nuclease mutant V66I/V99L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2011-05-02 |
| Detector | RIGAKU AFC9 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.450, 47.450, 63.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.140 - 1.601 |
| R-factor | 0.20971 |
| Rwork | 0.207 |
| R-free | 0.25152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.539 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.220 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.033 |
| Number of reflections | 15733 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 84.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 50mM phosphate buffer,pH 7.5 45% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
