4K0G

Crystal structure of human CLIC1 C24S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-29
Detector	ADSC QUANTUM 210r
Spacegroup name	C 1 2 1
Unit cell lengths	82.215, 82.728, 42.167
Unit cell angles	90.00, 116.43, 90.00

Refinement procedure

Resolution	27.194 - 1.400
R-factor	0.1563
Rwork	0.155
R-free	0.18060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k0m
RMSD bond length	0.005
RMSD bond angle	0.954
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.760	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.434
Number of reflections	49590
<I/σ(I)>	17.9	3.9
Completeness [%]	99.9	100
Redundancy	7.2	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2M Calcium acetate hydrate, 20% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K