4K08
Periplasmic sensor domain of chemotaxis protein, Adeh_3718
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.595, 50.595, 112.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.594 - 2.000 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.23770 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.675 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.030 | 0.196 |
| Number of reflections | 21845 | |
| <I/σ(I)> | 53 | 8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | Membfac-2 (12% PEG4000, 0.1M sodium acetate pH 4.6, 0.1 M zinc acetate) diluted by water; (membfac-2, 350 uL + water, 150 uL), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
