4K08
Periplasmic sensor domain of chemotaxis protein, Adeh_3718
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-23 |
Wavelength(s) | 0.97911 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 50.595, 50.595, 112.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.594 - 2.000 |
R-factor | 0.1972 |
Rwork | 0.195 |
R-free | 0.23770 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.675 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.020 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.030 | 0.196 |
Number of reflections | 21845 | |
<I/σ(I)> | 53 | 8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.8 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | Membfac-2 (12% PEG4000, 0.1M sodium acetate pH 4.6, 0.1 M zinc acetate) diluted by water; (membfac-2, 350 uL + water, 150 uL), VAPOR DIFFUSION, HANGING DROP, temperature 298K |