4K05
Crystal structure of a DUF1343 family protein (BF0371) from Bacteroides fragilis NCTC 9343 at 1.65 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-05 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.96109,0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.080, 88.410, 159.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.469 - 1.650 |
R-factor | 0.1508 |
Rwork | 0.149 |
R-free | 0.18260 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.469 | 1.710 | |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.066 | 0.025 | 0.498 |
Number of reflections | 91531 | 16983 | 15720 |
<I/σ(I)> | 10.11 | 28.6 | 1.7 |
Completeness [%] | 95.4 | 94.8 | 87.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M potassium formate, 20% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |