4JZP
Crystal structure of BAP31 vDED at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.610, 70.610, 80.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.570 - 2.100 |
| R-factor | 0.16313 |
| Rwork | 0.162 |
| R-free | 0.18020 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.959 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.570 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 13291 | |
| <I/σ(I)> | 24.28 | 3.99 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 292 | 100 mM Na citrate, 33% PEG400 and 200 mM LiSO4, VAPOR DIFFUSION, temperature 292K, pH 4.2 |
