4JZK

Crystal Structure of Adenylate kinase of E. Coli with ADP/AMP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9998
Spacegroup name	P 21 2 21
Unit cell lengths	72.960, 78.800, 83.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.883 - 1.630
R-factor	0.2164
Rwork	0.214
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.181
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.110	1.720
High resolution limit [Å]	1.630	1.630
Rmerge	0.111	0.395
Number of reflections	58505
<I/σ(I)>	5.6	1.5
Completeness [%]	97.0	82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	2.3 M ammonium sulfate and 100 mM imidazole at pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K