4JZA
Crystal structure of a Legionella phosphoinositide phosphatase: insights into lipid metabolism in pathogen host interaction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.280, 119.653, 133.533 |
| Unit cell angles | 90.00, 101.33, 90.00 |
Refinement procedure
| Resolution | 49.440 - 2.584 |
| R-factor | 0.19788 |
| Rwork | 0.196 |
| R-free | 0.23695 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 130.930 |
| High resolution limit [Å] | 2.580 |
| Rmerge | 0.099 |
| Number of reflections | 164803 |
| <I/σ(I)> | 10.96 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M HEPES (pH 7.0), 0.1M succinic acid, and 6.5% (wt/vol) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
