4JZ8
Carbamate kinase from Giardia lamblia bound to citric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.928, 86.401, 101.960 |
| Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
| Resolution | 46.300 - 2.100 |
| Rwork | 0.218 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3KZF AND 2WE4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.092 | 0.322 |
| Number of reflections | 67363 | |
| <I/σ(I)> | 7.9 | 2.9 |
| Completeness [%] | 99.6 | 97.5 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.4 M ammonium citrate dibasic, pH 5.0, 21% PEG3350, crystal soaked in 2 mM inhibitor disulfiram (inhibitor did not bind but unit cell dimension b shrank by 6%), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
