4JYP

crystal Structure of KAI2 Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	50.690, 53.210, 55.770
Unit cell angles	89.49, 89.76, 63.84

Refinement procedure

Resolution	26.488 - 1.300
R-factor	0.1699
Rwork	0.169
R-free	0.19060
RMSD bond length	0.006
RMSD bond angle	1.091
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.4.0)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	26.488
High resolution limit [Å]	1.300
Rmeas	0.109
Rpim	0.064
Total number of observations	261965
Number of reflections	115956
<I/σ(I)>	6.7
Completeness [%]	90.1
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	22% (w/v) polyethylene glycol 8000, 0.1 M Na+-PIPES (pH 6.5), 0.3 M NaBr and 2 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K