4JYO

Structural basis for angiopoietin-1 mediated signaling initiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 64 2 2
Unit cell lengths	80.968, 80.968, 187.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.18894
Rwork	0.186
R-free	0.24231
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z3s
RMSD bond length	0.022
RMSD bond angle	2.057
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.120	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	13192
<I/σ(I)>	24.1	3
Completeness [%]	98.9	99.3
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.0 M Na/K-tartate, 0.1 M Tris, 0.2 M LiSO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K